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Vector Laboratories
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bmk 220 - by Bioz Stars,
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Proteintech
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Dyckerhoff GmbH
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Unigene
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M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit
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Image Search Results
Journal: Redox Biology
Article Title: p47 phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis
doi: 10.1016/j.redox.2020.101569
Figure Lengend Snippet: ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.
Article Snippet: Immunoblotting assay involves antibodies against p47 phox (Santa Cruz Biotechnology); NOX1 and NOX2 (Proteintech); p67 phox (Abcam); NOXO1 (ABclonal Technology); Rac (Santa Cruz Biotechnology); Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology); PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology); VASP (anti-Ser157, Affinity Biosciences; anti-Ser239 and pan-VASP, Cell Signaling Technology); ERK1/2 (anti-Thr202/Tyr204 and pan-ERK1/2, Cell Signaling Technology); p38 MAPK (anti-Thr180/Tyr182, Cell Signaling Technology);
Techniques: Phospho-proteomics, Western Blot, Expressing